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477600
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KurzbeschreibungCarbohydrate Labeling & Analysis Kit
Ausführliche BeschreibungThis kit contains the reagents, buffers, and separation capillaries required to label, separate, and quantify oligosaccharides and monosaccharides released from glycoproteins. After enzymatic or chemical release, sugars are labeled with a fluorophore (APTS) at the reducing termini by reductive amination. The stoichiometry of labeling is such that only one APTS molecule is attached to each molecule of oligosaccharide or monosaccharide. These highly charged and fluorescent oligosaccharides are easily resolved in an electric field and detected by laser-induced fluorescence. This kit includes Carbohydrate Separation Buffer (Qty: 56 mL); N-CHO Coated Capillary 50 µm x 65 cm (Qty: 2); Labeling Reagents (Qty: 1); Labeling Dye (APTS); (Qty: 4 x 5 mL); Labeling Dye Solvent (Qty: 1 mL); Glucose Ladder Standard (Qty: 50 mg); and Quantitation/Mobility Marker (Maltose) (Qty: 20 mg).
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