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KurzbeschreibungSDS-MW Analysis Kit
Ausführliche BeschreibungThis kit is designed for the separation and sizing of protein utilizing SDS complexes as a replaceable gel matrix. The gel is formulated to provide an effective protein sieving range of approximately 10 to 225 kDa. Within this size range, the logarithm of protein molecular mass is linear with its reciprocal electrophoretic mobility, allowing the molecular weight of an unknown protein to be estimated from a standard curve of known protein sizes. This chemistry can also be used to effectively quantify the amount of protein and to determine the purity of a protein product. The kit includes: Bare-Fused Silica Capillary, 50 µm ID x 57 cm, Qty: 2; SDS-MW Gel Separation Buffer, Qty: 140 mL; SDS-MW Sample Buffer, 100 mM Tris-HCl, pH 9.0/1% SDS, Qty: 50 mL; SDS Protein Sizing Standard (10 to 225 kDa), 16 mg/mL Qty: 100 µL; Internal Standard, 10 kDa protein, 5 mg/mL Qty: 0.4 mL; Acidic Wash Solution, 0.1 N HCl Qty: 100 mL; Basic Wash Solution, 0.1 N NaOH Qty: 100 mL.
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