IgG Purity/ Heterogeneity Assay Kit

This kit allows for the assessment of purity and heterogeneity of proteins in both a reduced and non-reduced state. The methodology involves heat denaturing a specified concentration of IgG (both reduced and non-reduced) in the presence of SDS and separating these proteins by size using high-resolution capillary gel electrophoresis technology. This assay will detect impurities as low as 0.1% (0.01% if Laser-Induced Fluoresence (LIF) detection and dye are used) and includes an IgG protein control with a designated quantity of non-glycosylated heavy chain to test both the resolution and quantitation suitability of the assay prior to running unknowns. The kit includes Bare-Fused Silica Capillary, 50 µm ID x 57 cm (Qty: 2); SDS-MW Gel Buffer (Qty: 140 mL); SDS Sample Buffer, 100 mM Tris-HCl, pH 9.0/1% SDS (Qty: 50 mL); IgG Control Standard, 1 mg/mL in SDS sample buffer (Qty: 1 mL); Internal Standard, 10 kDa protein, 5 mg/mL (Qty: 0.4 mL); Acidic Wash Solution, 0.1 N HCl (Qty: 100 mL); and Basic Wash Solution, 0.1 N NaOH (Qty: 100 mL).

1.005,90 €

Für ein Produktangebot fügen Sie die Artikel, welche Sie erwerben möchten, Ihrem Warenkorb hinzu. Auf der Warenkorbseite können Sie Ihren Warenkorb ausdrucken. Speichern Sie die gedruckte Warenkorbansicht als PDF. Diese können Sie nun als Ihr Angebot verwenden.