IgG Purity and Heterogeneity Kit

The IgG Purity and Heterogeneity Kit is designed for use on the PA 800 Plus and is used to assess the purity and heterogeneity of IgG molecules in both a reduced and non-reduced state. The methodology involves heat denaturing a specified concentration of IgG (both reduced and non-reduced) in the presence of SDS. These products are separated using a replacable gel matrix within a capillary for separation. This kit will detect impurities as low as 0.1% and includes a standard, IgG control with a designated quantity of non-glycosylated heavy chain to test both the resolution and quantitation suitability of the assay prior to running unknowns. This kit provides reagent volumes for analysis of 100 samples and includes: • Separation Capillary, 67 cm x 50 µm ID Bare-Fused Silica, (2) • SDS Gel Separation Buffer, 140 mL • SDS Sample Buffer, 100 mM Tris-HCl, pH 9.0/1% SDS, 50 mL • IgG Control Standard, 1 mg/mL in SDS sample buffer , 1 mL • Internal Standard, 10 kDa protein, 5 mg/mL, 0.4 mL • Acidic Wash Solution, 0.1 N HCl, 100 mL • Basic Wash Solution, 0.1 N NaOH, 100 mL